Figure 4.

Catalytically important residues in ϵ–HOT. Top: Residues colored in red have large energetic contributions to transition state stability and/or are present in all transition states, residues in blue are active site residues. Reproduced from Cisneros, G. A., Perera, L., Schaaper, R. M., Pedersen, L. C., London, R. E., Pedersen, L. G., and Darden, T. A. (2009) Reaction Mechanism of the ε Subunit of E. coli DNA Polymerase III: Insights into Active Site Metal Coordination and Catalytically Significant Residues. J. Am. Chem. Soc.131, 1550–1556. Copyright 2009 American Chemical Society.⁶⁷