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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Vaccine. 2017 Oct 25;35(48 Pt B):6691–6699. doi: 10.1016/j.vaccine.2017.10.018

Figure 2. Replication of M2-83 mutants in MDCK cells and hNEC cultures.

Figure 2

(A) Plaque assays performed with indicated virus on MDCK cells. (B) Quantification of plaque diameter from 34–64 individual plaques per virus identified from 2–3 independent experiments. *p < 0.05. No conditions were statistically significant compared to rUd M2-WT. Low MOI multistep growth curves performed on (C) MDCK cells or (D) hNECs with the indicated viruses at 32°C. Data are pooled from two independent replicates each with n = 3 wells per virus (total n = 6 wells per virus). *p < 0.05 compared to rUd M2-WT (two-way ANOVA with Dunnett’s posttest). In (C) no time points were significantly different while in (D) rUd M2-A83M (48, 72, 96, and 120 HPI), rUd M2-A83K (24, 36, and 48 HPI) showed differences. Dotted line indicates limit of detection.