Fig 3.
Immunostaining of biotinylated lotus tetragonolobus lectin for detection of atubular glomeruli in Nep25+/DTR− and Nep25+/DTR+ mice kidneys at week 6 after DT injection. (A) Schema of method for counting atubular glomeruli. (B) Atubular glomeruli were increased in Nep25+/DTR+ vs. Nep25+/DTR− mice at week 6, but with increased frequency of atubular glomeruli after LMB2 injection. (C) WT-1+ cell density was lower in atubular glomeruli than in tubule-connected glomeruli in both groups at week 6. (D) LMB2 injection induced more severe glomerulosclerosis in tubule-connected glomeruli in Nep25+/DTR+ than in Nep25+/DTR− mice.