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COX2 and nNOS in kidney cortex in Nep25+/DTR− and Nep25+/DTR+ mice kidneys at week 6 after DT injection. (A) Nep25+/DTR+ only numerically enhanced COX2 expression vs Nep25+/DTR− mice. (B) nNOS, a key regulator of tubuloglomerular feedback at the macula densa, was stimulated by LMB2 injection, and both its mRNA and protein level were higher in Nep25+/DTR+ than Nep25+/DTR− mice after LMB2 injection.
