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Examples to show that making a CTF correction with an inappropriate value of defocus results in imperfect restoration of the image. Upper row: images of a point (i.e. a single-voxel) that were simulated for values of defocus that are specified by the numbers below each column. Lower row: corresponding images obtained after applying the CTF correction that is appropriate for a defocus value of 5000 Å. Scale bar = 10 Å.
