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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Microbiol. 2017 Oct 26;106(5):832–846. doi: 10.1111/mmi.13849

Figure 1. Effect of LytA on pneumococci in exponential growth phase in CY medium.

Figure 1

A. Growth curves of WT and ΔpgdA strains in the absence or in the presence of LytA at 10 μg/ml.

B. Growth curves of WT and Δadr strains in the absence or in the presence of LytA at 10 μg/ml.

C. Growth curves of Δadr strain in the presence of active LytA or inactive LytAE87Q forms, both proteins (final concentration of 10 μg/ml) were added at mid-exponential phase, OD595nm 0.3–0.4 as indicated by the arrow.

D. Complementation of the adr gene deletion. The pneumococcal Δadr or the WT strains were transformed with the Zn-inducible plasmid pJBadr containing a copy of adr or with the empty plasmid (pADG02). Active LytA (10 μg/ml) was added at mid-exponential phase (OD595nm 0.3–0.4, arrow).