Skip to main content
. Author manuscript; available in PMC: 2018 Dec 15.
Published in final edited form as: Anal Biochem. 2017 Oct 18;539:81–89. doi: 10.1016/j.ab.2017.10.014

Table 3.

Specific activity of hCE1, hCE2, hCE3, and FAAH towards 2-methyl-6-methoxy-3-pyridinyl amides with varying acyl chains. Substrate activities ([S] = 50 µM) were measured at 37°C in phosphate buffer (100 mM, pH = 8, 0.1 mg/mL BSA) containing 1% DMSO. Values represent the average of triplicates for a single experiment.

graphic file with name nihms915565u2.jpg
No. R x hCE1a hCE2b hCE3c Ratio
hCE1/hCE2
Specific activity (pmol.min−1.µg−1)
2 graphic file with name nihms915565t14.jpg 0 1.1 <1 <1 n/a
3 2 6.8 14 <1 0.5
4 4 53 36 2.3 1.5
5 5 180 28 3.9 6.4
1a 6 440 33 6.0 13
6 7 390 37 8.5 11
7 8 130 30 10 4.3
8 10 19 29 9.8 0.7
9 12 2.6 25 7.8 0.1
10 graphic file with name nihms915565t15.jpg 0 <1 <1 <1 n/a
11 1 <1 <1 <1 n/a
12 graphic file with name nihms915565t16.jpg 0 2.3 3.2 <1 0.7
13 1 2.9 <1 <1 n/a
14 2 6.3 7.1 <1 0.9
15 3 190 4.9 <1 38.8
16 4 93 18 <1 5.2
17 5 52 5.4 1.6 9.6
18 7 82 30 10 2.7
19 (4-MOMMP) graphic file with name nihms915565t17.jpg 0 450 13 <1 35
20 graphic file with name nihms915565t18.jpg 0 510 18 4 28
a

Values were calculated based on estimated of 25%.

b

Values were calculated based on estimated of 4%.

c

Values were calculated based on estimated of 3%.