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The absence of a microbiota does not affect cell death or microglial labeling in the prenatal hippocampus and hypothalamus. (A, C, E) AC3 and (B, D, F) Iba1 labeling in the PVN (A, B), CA1 oriens (C, D), and ARC (E, F) in conventionally colonized (CC) and germ-free (GF) mice on embryonic day (E) 18.5. N = 10 – 12 mice per group (A, C, E); N = 9 – 12 mice per group (B, D, F). - Effects of the absence of a microbiota were examined in the neonatal mouse brain.
