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. 2017 Sep 21;8(54):92004–92017. doi: 10.18632/oncotarget.21122

Figure 4. mPGES-1 and EP1-4 mRNAs and proteins expression in primary cultured hippocampus neuron.

Figure 4

(A) The expressions of mPGES and EPs mRNA were measured by RT-PCR. The relative mRNA level of mPGES and EPs were standardized to endogenous β-actin mRNA for each sample. Aluminum administration caused the significant increase of mPGES, EP1, EP2 AND EP4 levels and decrease of EP3 level compared with the control group. (B) The expressions of mPGES and EPs proteins were measured by WB. The relative protein levels of mPGES and EPs were standardized to endogenous β-actin protein for each sample. Al administration caused the significant increase of mPGES, EP1 and EP2 levels and decrease of EP3 level compared with the control group. Values were mean ± SD (n = 3, *P < 0.05, **P < 0.01 vs. control group, one-way ANOVA with Dunnett's multiple comparisons).