Figure 4. KRAS restores cell proliferation which is suppressed by silibinin.

T24 and UM-UC-3 cells were treated with 10 μM silibinin after transfection with vector only or vector expressing KRAS for 48 hours. KRAS expression was analyzed by Western blot (A). Cell viability (B) and cell invasion (C) were evaluated at 48 hours after treatment with 10 μM silibinin. Two-tailed Student's t-test (B, C). Percentages in control-treated cells were defined as 100% (B, C). ^* p < 0.05.