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. 2017 Sep 8;8(54):92032–92042. doi: 10.18632/oncotarget.20734

Figure 5. Silibinin suppressed histone H3 lysine 4 (H3K4) trimethylation (H3K4me3) and H3 acetylation (AcH3) and down-regulated KRAS expression.

Figure 5

(A) Relative KRAS mRNA expression after 10 μM silibinin treatment for 48 hours. (B) Schematic representation of the promoter region of the human KRAS gene and primer set location. (C) ChIP assays were performed to detect Ras levels at multiple primer sets (ChIP 1-ChIP 6). (D) The relative enrichment of H3K4me3 and AcH3 at ras primer-4 (ChIP-4) decreased after silibinin treatment for 48 hours. Two-tailed Student's t-test (A). Expression levels in control-treated cells were defined as 1 (A). *p < 0.05, ***p < 0.001.