Figure 6. Silibinin down-regulates expression of oncogenic lncRNAs: HOTAIR and ZFAS1.

Expression of long non-coding RNA HOTAIR (A) and ZFAS1 (B) after treatment of T24 and UM-UC-3 cells with silibinin (10 μM) for 4 hours as determined by real-time PCR (mean ± SD). GAPDH was used as an internal control. Data were obtained from three independent experiments performed in triplicate. Two-tailed Student's t-test (A, B). Expression levels in control-treated cells were defined as 1 (A, B). ^*p < 0.05, ^***p < 0.001.