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. 2017 Sep 14;8(54):92143–92156. doi: 10.18632/oncotarget.20922

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Copyright: © 2017 Ssadh et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Cell surface expression of CD74 (upper panel, (A) and CD44 (lower panel, (B) on CAMA-1, MDA-MB-231, MDA-MB-435, Raji and HeLa cells. All cells were cultured in the appropriate media and were acquired by flow cytometry using By2 (anti-CD74) and 156-3C11 (anti-CD44). Empty histograms represent the aforementioned cell lines labeled with anti-CD74 and anti-CD44 antibody. Black-filled histograms show the isotypes serving as negative controls. Cells were labelled using FITC-labelled secondary anti-mouse antibody. Expression levels were analyzed by flow cytometry (Aria cell sorter) and FlowJo 8.8.6 software was used to analyze the data. Mean fluorescence intensity (MFI) values were measured based on geometric means. Mouse IgG was used as a negative control. Data are representative of three independent experiments.