Figure 5. Effect of Verrucarin J on generation of ROS.

A2780 cells were treated with various concentrations of VJ for 24 h. ROS generation was assayed by incubation of cells with H₂DCFA for 30 min. Cells were rinsed with PBS and stained with DAPI for nuclei. The cells were examined using confocal microscope and photographed. Green cells show the production of ROS. A significant high level of ROS was observed in cells treated with 5 nM or 10 nM of VJ compared to vehicle treated cells (0 nM VJ). Results shown are representative of three independent experiments.