Figure 6. Effect of Verrucarin J on elimination and tumorigenic potential of ALDH1⁺ CSCs.

(A) = A2780 cells were treated with various concentrations of VJ. After 24 h of treatment cells were harvested with trypsin free cell dissociation buffer. ALDH1⁺ cells were analyzed by using Aldefluor kit followed by FACS analysis. The data shown is mean ± SD of three independent experiments. ^* represents significance at p ≤ 0.05. (B) = ALDH1⁺ cells were isolated from A2780 cell line and plated on ultralow attachment plates. After 24 h of treatment, ALDH1⁺ spheroids were treated with various concentrations of VJ. After 72 h of treatment, spheroids were examined under phase contrast microscope, counted and photographed. (C) = quantitative analysis of spheroids on treatment with VJ. The data shown is mean ± SD of three independent experiments. ^* represents significance at p ≤ 0.05.