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| Gene | Forward | Reverse |
|---|---|---|
| ALDH1 | GCACGCCAGACTTACCTGTC | CCACTCACTGAATCATGCCA |
| LGR5 | GCAAACCTACGTCTGGACAA | TGATGCTGGAGCTGGTAAAG |
| NANOG | ACTCTCCAACATCCTGAA | TTCTGCCACCTCTTAGAT |
| OCT4 | CGCTGGCTTATAGAAGGT | ACAGGTGTCATAAGAATGGATA |
| NOTCH1 | TCAGCGGGATCCACTGTGAG | ACACAGGCAGGTGAACGAGTTG |
| WNT1 | CTCTCTTCTTCCCCTTTGTC | AACTCGTGGCTCTGTATCC |
| SECURIN | TCGAATTCGACCTGCAATAATCCAGAAT | GCTTTAACAGTCTTCTCAGT |
| GAPDH | TGATGACATCAAGAAGGTGGT | TCCTTGGAGGCCATGTGGGCC |
