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(A-C) Ishikawa cells transfected with siPrx3 or siCont for 48 hours, and then treated with doxorubicin or vehicle for 24 hours. Mitochondrial membrane potential (A), mitochondrial ROS levels (B), and calcium levels (C) measured using FACSCantoII analysis, after staining with TMRE, Mito-Sox, and Rhod-2AM. (D and E) Ishikawa cells transfected with siRNA against Prx3 or siCont; OCR (D) and mitochondrial ATP levels (E).
