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. 2017 Oct 10;8(54):93039–93050. doi: 10.18632/oncotarget.21750

Figure 3. Celastrol induced autophagy in HCC cells.

Figure 3

(A) Electron microscopy images showing ultrastructural features of a representative control cell and the morphological features of autophagy in Bel7402 cells treated with 0.625 μM, 1.25μM and 2.5μM celastrol for 24 h. Cells were observed at different magnifications (x 5 000 and x 20 000). (B) Bel7402 cells were fixed, permeabilized, and successively incubated with anti-LC3B antibodies and a secondary antibody conjugated Alexa Fluor® 647. The nuclei were stained with DAPI. (C) LC3B expression in HepG2 and Bel7402 cells after treatment with 1.25 and 2.5 μM celastrol for 24 hours.