Fig. 6.

Representative histological photographs of the repaired areas implanted with tissue-engineered scaffolds of g-HA/PLGA (a) and PLGA (b) with autologous MSCs, and the pure defect (c) at eight weeks post-surgery; typical histological micrographs (d, e) of the interface between the implant and the newly formed bone at eight weeks post-surgery

(a, b) Cross-sections through the center of repaired areas are shown. Photos were taken using a Fujifilm FinePix S602 digital camera with 6×optical zoom. (c) Vertical sections through the pure bone defect with no material replacement are shown. The photos were taken using a light microscope. The samples were decalcified and slices were treated with H&E staining and Masson’s trichrome staining. (d) Implant of tissue-engineered g-HA/PLGA, acidophilous osteoclast and basophilic osteoblast. (e) Implant of tissue-engineered PLGA. The gap of the PLGA significantly parceled fibrous tissues. The samples were decalcified and slices were treated with H&E staining and Masson’s trichrome staining. Scale bars are 1000 (a), 1200 (b), 250 (c), 25 (d), and 100 (e) μm