Table 7.
Characteristics of 11 microsatellite loci and their genetic diversity among the wild L. ruthenicum
| Primer | GenBank No. | Sequence (5'→3') | Repeat motif | Size (bp) | T a (°C) | F is | H o | H e | N A | PIC |
| U20 | KY284848 | F: CCCACTTCTCAAAAATGGTACAC | (ATT)6 | 88 | 60.0 | 0.058 | 0.544 | 0.481 | 4 | 0.4232 |
| R: ATAGTTGCCAACAAACCCTTCTT | ||||||||||
| U21 | KY284849 | F: GGATGAAGAAGAAGAGGATGACA | (AAT)6 | 126 | 59.9 | 0.042 | 0.324 | 0.458 | 5 | 0.4174 |
| R: CTTCTCAAAAATGGTACACTGCC | ||||||||||
| U23 | KY284850 | F: CTACTTCCATTTGTGGAAAGCTG | (TGC)6 | 164 | 60.0 | 0.179 | 0.576 | 0.667 | 4 | 0.6120 |
| R: TAGCCAGTCTAATCTTCGGTTTG | ||||||||||
| U25 | KY284851 | F: CAGGAAGGAGAAGAGTCTGATGA | (GCA)5 | 90 | 60.1 | 0.011 | 0.064 | 0.115 | 3 | 0.1112 |
| R: TTATCATTAACGGCTTCCATTTG | ||||||||||
| U26 | KY284852 | F: AATGGGGAAAGGTAAAGGAAGTT | (GTA)6 | 156 | 60.1 | 0.135 | 0.576 | 0.539 | 4 | 0.4701 |
| R: CCTTGTGGAATTTTACTTTCCAAT | ||||||||||
| U27 | KY284853 | F: CCACCCAGATAGTGGTGGTAATA | (GAA)6 | 108 | 59.8 | 0.072 | 0.380 | 0.379 | 5 | 0.3403 |
| R: GCTGATGTTTTCACATTTGTCAC | ||||||||||
| U31 | KY284854 | F: TAGGGTTTGAGGGTTTGAAGAAT | (CAC)5 | 109 | 59.4 | 0.076 | 0.164 | 0.202 | 3 | 0.1918 |
| R: ATTATTATGGCTTCTTCACCTGG | ||||||||||
| U36 | KY284855 | F: CTACCACTCCAACGTGTACCAAT | (CAA)6 | 138 | 60.1 | 0.099 | 0.536 | 0.607 | 4 | 05489 |
| R: TTCTTGCTCTAATTCTGAAACCG | ||||||||||
| U42 | KY284856 | F: GTCTCCATTTTACCCCTACCAAG | (ATT)6 | 150 | 60.2 | 0.135 | 0.316 | 0.726 | 4 | 0.6750 |
| R: TTTGCAAATAAAATGCGATTATTG | ||||||||||
| U46 | KY284857 | F: ATGAAGGCAATATTTAGGGCAGT | (TTG)7 | 153 | 60.3 | 0.049 | 0.132 | 0.270 | 3 | 0.2525 |
| R: CAATTTCATATTTGTGCTCTGCAT | ||||||||||
| D1 | KY284858 | F: TTCCAAGAACATTAGCACAAACA | (TTGGCT)4 | 136 | 59.7 | 0.027 | 0.840 | 0.576 | 4 | 0.4868 |
| R: TGGCACTTGTCCTAGTCCTAAAC |
T a: annealing temperature; F is: inbreeding coefficient; H o: observed heterozygosity; H e: expected heterozygosity; N A: allele numbers; PIC: polymorphism information content