Figure 1.

Nippostrongylus brasiliensis (Nb) induces the recruitment and activation of neutrophils in the skin. C57BL/6 mice were injected intradermally into the ear with 600 AF488⁺ Nb or PBS. The resulting inflammatory response in blood and skin was analyzed by flow cytometry at the indicated time points. Each symbol represents one mouse. (A) Proportion of neutrophils (CD11b^hi Ly6G⁺) in peripheral blood leukocytes (PBL). Data are pooled from two independent experiments. (B) Recruitment of leukocyte populations in the ear skin at 24 h. Leukocytes were identified as CD45⁺ cells, neutrophils and monocytes were identified according to the gating in panel C. Data are from one of three experiments that gave similar results. (C) Representative dot plots depicting the gating of ear skin CD45⁺ populations expressing Ly6G (neutrophils), high Ly6C (monocytes), and AF488 as a measure of Nb uptake. (D) Pie chart showing the relative proportion of AF488⁺ populations in skin, identified as in (C). Monocyte-derived DCs (moDCs) are defined as Ly6G⁻ Ly6C^hi CD11b^hi CD11c⁺ MHCII⁺, and CD11b⁺ dendritic cells (DCs) are Ly6G⁻ Ly6C⁻ CD11c⁺ MHCII⁺ CD11b⁺ CD326⁻. Data are from one of three experiments that gave similar results. Bar graphs show mean ± SEM. Statistical analyses used the Mann–Whitney test. NS: not significant; *p < 0.05; **p < 0.01; ****p < 0.0001. (B) Symbols close to the legend indicate a comparison of the same population between the two groups.