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. 2017 Nov 16;8:1575. doi: 10.3389/fimmu.2017.01575

Figure 6.

Figure 6

Toll-like receptor 4 (TLR4) enhances the development of CD4+IL4+ T cells after Nippostrongylus brasiliensis (Nb) injection. C57BL/6 or TLR4 KO mice were injected intradermally into the ear with 150 Nb from different preparations. T cell responses were measured in the draining lymph node by intracellular cytokine staining and flow cytometry 7 days after Nb injection. Each dot corresponds to one mouse. Data are from one of at least two repeat experiments that gave similar results. (A) T cell cytokine response in C57BL/6 mice injected with Nb, or Low Endotoxin (LE, <5 Endotoxin U/mL) Nb, or Low Endotoxin Nb preparations (LE-Nb) plus 10,000 formalin-fixed E coli. (B) T cell cytokine response in C57BL/6 (WT) and TLR4 KO mice injected with Nb together with IFNAR1-blocking antibodies or isotype control. Bar graphs show mean ± SEM. Statistical analyses used the ANOVA with Tukey’s multiple comparisons test. Ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.