Fig. 5.

R1288 of Ash1 is critical for Mrg15 binding capacity and Mrg15-mediated Ash1 activation. a Schematic map shows the Flag-Ash1 C-terminal truncations used to determine the Mrg15 interaction domain. MID, Mrg15-interacting domain. b Co-IP results show Ash1 MID is essential for the Mrg15 interaction. c Co-IP results show that the R1288A mutation greatly weakens the interaction between Ash1C and Mrg15. d Histone methyltransferase assay results show that Mrg15-mediated Ash1 activation was severely compromised by the R1288A mutation