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. 2017 Nov 16;7:475. doi: 10.3389/fcimb.2017.00475

Figure 2.

Figure 2

Optimization of fluorescent protein production using a synthetic transcriptional terminator. Bacteria containing constructs with and without a synthetic transcriptional terminator (TT) were grown in 96-well plates and GFP fluorescence (A) or mCherry fluorescence (B) was measured at 2.5 h of growth (Mean ± SD, n = 3). AU, arbitrary units (C,D) as in Figure 1 relative promoter units were obtained by normalizing to ProA (RPUA) for GFP (C) or mCherry (D) at 1.5 and 2.5 h time points. (E–H) Flow cytometry analysis of bacteria harboring the indicated plasmids. Bacteria were harvested at early log (2 h), late log (3.5 h), early stationary (4 h), and late stationary (5 h) phases. Shown are representative histograms at the indicated time points for GFP constructs (E) or mCherry constructs (G). Fluorescence intensities of GFP (F) and mCherry (H) were plotted at each time point (Mean ± SD, n = 3).