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. 2017 Nov 16;7:475. doi: 10.3389/fcimb.2017.00475

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Copyright © 2017 Cooper, Chong, Starr, Finn and Steele-Mortimer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Titration of GFP production in Salmonella can eliminate plasmid associated invasion defects. (A) Invasion assay in HeLa cells. Values were normalized to bacteria containing no plasmid (Mean ± SD, n = 3). (B) Replication assay in HeLa cells (Mean ± SD, n = 3). (C) GFP fluorescent intensities of intracellular bacteria at 1.5 hpi. Shown is data from one representative experiment; each dot represents one bacterial cell. The means are indicated. The threshold was set at three-fold the mean intensity of bacteria with no plasmid (dotted line). ^*Signifies P-value ≤ 0.05.