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. 2017 Nov 16;7:475. doi: 10.3389/fcimb.2017.00475

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Copyright © 2017 Cooper, Chong, Starr, Finn and Steele-Mortimer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Design of the bidirectional environmental sensor plasmids. (A) Plasmid map of pCHAR-ProB.mCherry constructs. mCherry expression is driven by ProB; GFP expression is controlled by the glucose-6-phosphate (G6P) responsive promoter, PuhpT. (B,C) Induction of GFP fluorescence is G6P dependent. Late-log phase bacteria harboring pCHAR1-ProB.mCherry or Pnull-gfp (B) or pCHAR2-ProB.mCherry (C) were treated with 0–20 μM G6P and GFP (left) and mCherry (right) fluorescence was monitored in a plate-reader at 10 min intervals. Shown is data representative of three independent experiments with mean ± SD from triplicate samples.