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. 2017 Nov 16;8:932. doi: 10.3389/fphys.2017.00932

Figure 2.

Figure 2

MicroCT analyses of adult molar and incisor enamel. First column: first molars in mid sagittal plane. Middle column: Incisor enamel in coronal plane through the distal root of the first molar, representing early maturation stage. Last column: Incisor enamel in coronal plane adjacent and mesial to the first molar, representing maturation stage enamel. White arrowheads point to mineralized enamel layers visible in molars and incisors. WT seen in last row (S–U); (P–R) AmelxKO with little enamel on molars and no enamel in the incisor enamel. (A–C) homozygous triple transgene M180Tg/CTRNCTg/LRAPTg/KO++, showing the thinnest enamel layer compared to all samples shown on both molars and incisors. (D–F) Hemizygous triple transgene M180Tg/CTRNCTg/LRAPTg/KO+ and (M–O) hemizygous double transgene M180Tg/LRAPTg/KO+ with thicker enamel than all other transgene phenotypes shown, but thinner than WT. (G–I) double transgene M180Tg/CTRNCTg/KO++ with a thin layer of enamel as is also seen in (J–L) M180Tg/CTRNCTg/KO+. Ectopic depositions (orange arrowheads) were visible in incisors of homozygous transgenes M180Tg/CTRNCTg/LRAPTg/KO++ and M180Tg/CTRNCTg/KO++ (A–C, G–I). Scale bars, 500 μm.