Table 2.

Mouse genotypes (KO and transgenic status) as determined by PCR for genotyping for endogenous amelogenin and qPCR for measuring relative copy number of the three transgenes, using transgene-specific probes against M180Tg, CTRNCTg, and LRAPTg.

Genotypes	AmelxKO	M180Tg (180aa)	CTRNCTg (167aa, no C-terminus)	LRAPTg (59aa, C- and N-only)
1: M180Tg/CTRNCTg/LRAPTg/KO++	−/o or −/−	++	++	++
2: M180Tg/CTRNCTg/LRAPTg/KO+	−/o or −/−	+	+	+
3: M180Tg/CTRNCTg/KO++	−/o or −/−	++	++	–
4: M180Tg/CTRNCTg/KO+	−/o or −/−	+	+	–
Control: M180Tg/LRAPTg/KO+	−/o or −/−	+	–	+
Control: AmelxKO	−/o or −/−	–	–	–
Control: WT	+/o or +/+	–	–	–