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. 2017 Nov 20;8:1614. doi: 10.1038/s41467-017-01737-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Genetic deletion of miR-29 in a mouse model for left ventricular pressure overload. a Expression of miR-29 family members in left myocardium from wildtype (WT), miR-29 ab1 ^−/− b2c ^+/− and miR-29 ab1 ^+/+ b2c ^−/− mice; n = 4–6 mice/group. b Echocardiographic analysis of fractional shortening as a measure of left ventricular function; n = 4–10 mice/group. A Student’s t-test was used to calculate the P values. c (Left) Representative stainings of myocardial tissue from WT or knockout mice (tissue fixation 21 days after sham surgery or transverse aortic constriction, TAC) by hematoxylin/eosin and Sirius Red/Fast Green stainings. Scale bar: 2 mm. (Right) Ratio between heart weight and tibia length (HW/TL) as a measure of cardiac hypertrophy; n = 6–14 mice/group. P values were determined by two-way ANOVA followed by Bonferroni’s post hoc test. d (Left) Representative wheat germ agglutinin (WGA)-staining of midventricular sections to assess hypertrophy of cardiac myocytes. Scale bar: 50 µm. (Right) Quantitative analysis; n = 5–8 mice/group. P values were calculated using two-way ANOVA followed by Bonferroni’s post hoc test. e (Left) Representative image sections from Sirius Red/Fast Green-stained myocardium of the indicated groups and (Right) quantitative analysis of fibrosis; n = 3–11 mice/group. P values were determined by two-way ANOVA followed by Bonferroni’s post hoc test. f Real-time PCR quantification of markers for cardiac remodeling in left ventricular tissue from WT, miR-29 ab1 ^−/− b2c ^+/− and miR-29 ab1 ^+/+ b2c ^−/− mice. Collagen mRNAs and the following markers of cardiac myocyte hypertrophy were assessed: Nppa, atrial natriuretic peptide; Myh7/Myh6, ratio of mRNAs encoding β- and α-myosin heavy chain. Tissues were collected 21 days after TAC surgery; data are from 4 to 9 independent experiments, with 2 replicates each. WT TAC means were compared to that of miR-29 ab1 ^−/− b2c ^+/− and miR-29 ab1 ^+/+ b2c ^−/− mice by a one-way ANOVA followed by a Bonferroni’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 for all panels. All quantitative data are reported as means ± SEM