Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 21;8:1638. doi: 10.1038/s41467-017-01807-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — HSF1–PARP13–PARP1 enhances DNA damage-induced gene expression. a Venn diagram showing genes upregulated by HSF1 knockdown and substitution with hHSF1-T20A in HeLa cells (fold change > +1.7; P < 0.05; n = 3). b Gene ontology enrichment analysis of 71 genes upregulated by substitution with hHSF1-T20A. The P-values and gene names associated with each functional category are shown. Names of DNA-damage-inducible genes are indicated in red. c Venn diagram showing genes upregulated by DOX treatment and by substitution with hHSF1-T20A (fold change > +1.7; P < 0.05; n = 3). d Expression of DNA-damage-inducible genes in unstressed condition. RT-PCR analysis of five genes was performed using cells knocked down for HSF1, PARP1, PARP13, or both PARP1 and PARP13 (double-KD) (upper). Cell extracts were also subjected to immunoblotting (lower). e Expression of GADD genes in cells expressing HSF1 mutants. Cells, in which endogenous HSF1 was replaced with each protein, were treated with DOX for the indicated periods. GADD mRNA levels were quantified by RT-qPCR (n = 3) (upper). Mean ± s.d. is shown. Asterisks indicate P < 0.01 by ANOVA. Extracts from cells before and after treatment were subjected to immunoblotting (lower). f Expression of GADD34 gene in cells expressing PARP13 mutants. Cells, in which endogenous PARP13 was replaced with each mutant, were treated with DOX for the indicated periods. GADD34 mRNA levels were quantified by RT-qPCR (n = 3) (left). Mean ± s.d. is shown. Asterisks indicate P < 0.01 by ANOVA. Extracts from cells before and after the treatment were subjected to immunoblotting (right)