Fig. 4.

Validation of enzyme digestion and proximity ligation. a The enzymes HaeIII, MboI, and HindIII are applied for BL-Hi-C library construction with the same two-step ligation protocol. The distances from BL-Hi-C peaks to CTCF or RNAPII ChIP-seq peaks, measured from the centers, are investigated. The y-axis refers to the average BL-Hi-C peak depth, which is normalized by 1 M sequenced reads. b One-step (green) or two-step (blue) proximity ligation is applied to the construction of BL-Hi-C libraries with the same HaeIII enzyme digestion. The x-axis shows the percentage of BL-Hi-C peaks ranked by q-value for each library. The y-axis shows the percentage of peaks that overlap with the public CTCF or RNAPII ChIP-seq data