Skip to main content
. 2017 Nov 21;37(6):BSR20171039. doi: 10.1042/BSR20171039

Figure 7. Suppression of POPDC1 is overcome by SP-8-Br-cAMPS and rescues inhibited cell migration and proliferation in breast cancer cells.

Figure 7

(A) Boyden chamber assay analysis of the effects of 60 µM Sp-8-Br-cAMPS on MCF7 (n=4), MDA231 (n=4) and SKBR3 (n=4) breast cancer cell migration following POPDC1 suppression with siRNA. Boyden chamber assay was performed over a 3-h incubation period to allow cell migration across the polycarbonate membrane; scale bars = 250 μm. (B) Alamar Blue assay analysis of the effects a two-phase 60 µM Sp-8-Br-cAMPS treatment on the proliferation of MCF7 (n=5), MDA231 (n=5) and SKBR3 (n=5) cells following POPDC1 suppression with siRNA. Cells were transfected for 36 h and starved overnight prior to drug treatment. Cells were treated with 60 µM Sp-8-Br-cAMPS treatment for 1 h prior to treatment replacement with a fresh 60 µM Sp-8-Br-cAMPS treatment solution and 24-h incubation. Alamar Blue assay was performed by incubating cells in 10% AlamarBlue® dye for 4 h. Mean % migration and mean % proliferation values were compared using an unpaired t-test. Mean values presented ± SEM.