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. 2017 Nov 21;37(6):BSR20170973. doi: 10.1042/BSR20170973

Figure 5. IL-12 induced the activation of NF-κB signaling pathway.

Figure 5

(A) hPDLFs were pretreated with 10 µM of PDTC for 1 h, followed by incubation with 10 ng/ml of IL-12 for, 6, 12, and 48 h, and total proteins were then extracted. The protein levels of IκBα, p-IκBα, NF-κB P65, and p-NF-κB P65 were subjected to Western blot analysis. β-actin served as the loading control. (B) hPDLFs were pretreated with 10 µM of PDTC for 1 h, and incubated with 10 ng/ml of IL-12 for 6, 12, and 48 h. The cytoplasmic and nuclear proteins were respectively prepared as described in the ‘Materials and methods’ section. Cytoplasmic and nuclear NF-κB P65 protein were analyzed with Western blotting. β-Actin and Lamin A expression served as the internal control of cytoplasm and nucleus, respectively. The fold increase in protein expression was normalized to β-actin or Lamin A. The presented Western blot image is a representative result of three independent experiments. Data are the means ± S.E.M. (n=3). **P<0.01 compared with the control (0 ng/ml of IL-12); #P<0.05 compared with the IL-12 group.