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. 2017 Nov 20;7:15852. doi: 10.1038/s41598-017-15626-9

Figure 2.

Figure 2

Overexpression of miR-17-92 cluster promotes proliferation of DF1 and ICPA-1 cells. (a) Effect of overexpression of miR-17-92 cluster on chicken cell proliferation. DF1 and ICPA-1 cells were transfected with either pcDNA3.1-miR-17-92 or the empty vector pcDNA3.1, and cell proliferation was assessed at the designated time points using the CCK-8 kit. (b and c) Effect of overexpression of miR-17-92 cluster on expression of proliferation marker genes in DF1 cells (b) and ICPA-1 cells (c). Cells were transfected with either pcDNA3.1-miR-17-92 or pcDNA3.1 vector, total RNA was isolated, and the gene expression of Cyclin D1, Ki67, PCNA and E2F1 were assessed at 48 h post-transfection using qRT-PCR. Gene expression was normalized to NONO mRNA level. Fold change is relative to pcDNA3.1 vector at 48 h after transfection. All data are representative of three independent experiments and shown as the mean ± SEM. *p < 0.05; **p < 0.01; determined by two-tailed Student’s t-test.