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. 2017 Nov 20;7:15852. doi: 10.1038/s41598-017-15626-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of MAP3K2 inhibits chicken cell proliferation. (a and b) Effect of overexpression of MAP3K2 on proliferation of DF1 (a) and ICPA-1 cells (b). The cells were transfected with pCMV-HA-MAP3K2 or pCMV-HA vector, and cell proliferation was assayed at designated time points after transfection using the CCK-8 kit. (c–h) qRT-PCR analysis of Cyclin D1, PCNA, Ki67 or E2F1 in DF1 (c–e) and ICPA-1 cells (f–h). The cells were transfected with either pCMV-HA-MAP3K2 or pCMV-HA vector, and at designated time points after transfection, total RNA was isolated, and gene expression was assessed using qRT-PCR analysis. Gene expression was normalized to NONO mRNA level. Fold change is relative to pCMV-HA vector at 24 h after transfection. All data are representative of three independent experiments and shown as the mean ± SEM. *p < 0.05; **p < 0.01; determined by two-tailed Student’s t-test.