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. 2017 Nov 20;7:15852. doi: 10.1038/s41598-017-15626-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MAP3K2 overexpression does not induce cell apoptosis. (a and b) Effect of MAP3K2 overexpression on cell apoptosis. DF1 cells (a) and ICPA-1 cells (b) were transfected with either pCMV-HA-MAP3K2 or pCMV-HA vector, and at 48 h after transfection, cell apoptosis was assessed using the Annexin V-FITC/PI kit. The results are representative of three independent experiments. (c) The quantification of cell apoptosis in DF1 and ICPA-1 cells. All data are representative of three independent experiments and shown as the mean ± SEM, and ns indicates no significance; determined by two-tailed Student’s t-test.