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. 2017 Nov 20;7:15852. doi: 10.1038/s41598-017-15626-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of overexpression of miR-17-92 cluster and MAP3K2 and knockdown of MAP3K2 on c-Myc expression in DF1 and ICPA-1 cells. DF1 cells (a–c) and ICPA-1 cells (d–f) were transfected with the designated plasmids or siRNAs; at 24 and 48 h after transfection, total RNA was isolated, and c-Myc expression was detected using qRT-PCR. Gene expression was normalized to NONO mRNA level. Fold change is relative to either pcDNA3.1 vector, pCMV-HA vector or siNC at 24 h after transfection. All data are representative of three independent experiments and shown as the mean ± SEM. *p < 0.05; **p < 0.01; determined by two-tailed Student’s t-test.