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. 2017 Nov 20;7:15826. doi: 10.1038/s41598-017-15870-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Infection of HGF into MSCs and the effect on MSC growth and apoptosis after. 12 hours of hypoxia. (A) Flow cytometry analysis of Annexin V/PI was carried out in MSCs. (B) Apoptosis rate was calculated according to Annexin V/PI flow cytometry analysis. (C) MTT curve of MSCs infected with Ad-HGF or vector in normoxic or hypoxic conditions. (D) Representative Western blot and level of Bcl-2, Bax and caspase-3. Data are mean ± SEM. **P < 0.01 vs. MSCs-normoxic, ^# P < 0.5, ^## P < 0.1 vs. Ad-EGFP-MSCs-hypoxic. n = 3, One-way ANOVA, then followed by post-hoc Tukey’s test for multiple comparisons.