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. 2017 Nov 20;7:15878. doi: 10.1038/s41598-017-15972-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HIF-1α shRNA on albumin-induced changes in the immunostaining of α-SMA, FSP-1 and E-cadherin. Upper panel: Representative confocal images showing the immunostaining of α-SMA, FSP-1 and E-cadherin. Lower panel: Summarized integrated optical intensity of the fluorescent staining. Ctrl, cells treated with control plasmids. Albumin, cells treated with albumin + control plasmids. Albumin + HIF-1α shRNA, cells treated with albumin + plasmids expressing HIF-1α shRNA. n = 6 batches of cells, *P < 0.05 vs. other groups.