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. 2017 Nov 20;7:15878. doi: 10.1038/s41598-017-15972-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PHD2 transgene or PHD2 shRNA on the mRNA levels of PHD2 and the protein levels of HIF-1α, collagen I and TIMP-1. (A) The relative mRNA levels of PHD2 by Real-time RT-PCR analysis. (B) Protein levels of HIF-1α, collagen I and TIMP-1. Upper panel: Representative gel documents. Lower panel: Summarized data showing band intensity ratio of HIF-1α, collagen I and TIMP-1 to β-actin normalized to the value in Ctrl. Ctrl, cells treated with control plasmids. PHD2, cells treated with plasmids expressing PHD2. PHD2 shRNA, cells treated with plasmids expressing PHD2 shRNA. n = 6 batches of cells, *P < 0.05 vs. other two groups.