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. 2017 Sep 15;250(6):629–639. doi: 10.1007/s00232-017-9988-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Quantification of aquaglyceroporins levels in the plasma membrane. a Quantitative Western blot analyses of aquaglyceroporins present in the plasma membrane. Protein content in plasma membrane fractions were analyzed by SDS-PAGE and Western blotting detecting Pma1 membrane marker and the polyhistidine-tag of the AQPs. Note that the typical aquaporin migrates in the gel as different mono/oligomers. b Quantification of the aquaglyceroporins present in the plasma membrane (His-signal divided by Pma1-signal), values are mean, and error bars denote SD