Fig. 4.

Applying SFM to measure cell shrinkage as indicative for water efflux. a Cells were subjected to a hyperosmotic shock causing outward movement of water. Normalized Light Scatter Intensity (NLSI) was plotted over time (s). b Water exchange rate constant (k) for aqy1Δagp1Δ, aqy1Δagp1Δ + hAQP3, aqy1Δagp1Δ + hAQP7, aqy1Δagp1Δ + hAQP9, values are mean of triplicates, and error bars denote SEM. Statistical significance was investigated with one-way ANOVA (F(3,8) = 14.38, p < 0.01), and Tukey’s multiple comparisons test rendered statistical significant difference (p < 0.05) between aqy1Δagp1Δ and aqy1Δagp1Δ + hAQP7 and non-significant differences comparing aqy1Δagp1Δ with aqy1Δagp1Δ + hAQP3 and aqy1Δagp1Δ + hAQP9