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. 2017 Nov 20;7:15850. doi: 10.1038/s41598-017-16199-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,D) Effects of chalcones N9, N17 and N23 on phenotypic characteristics and cell death of HN30 cells. The HN30 cells were treated with the chalcones N9 (15.61 µM), N17 (14.27 µM) and N23 (17.22 µM), for 48 h. (A) Control cells. (B) Cells treated with chalcone N9. (C) Cells treated with chalcone N17. (D) Cells treated with chalcone N23. The first and the second columns represent all cells or the supernatant of the cells analyzed by flow cytometry, respectively. The third column shows the morphology of the cells on microscopy and the fourth column the cell staining with DAPI. The white arrows represent cells in apoptosis. The last column shows the NMA analysis. N: Normal nuclei, Ir: irregular, LR: Large Regular; LIr: Large Irregular; SR: Small and Regular; S: Small.