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. 2017 Nov 20;7:15850. doi: 10.1038/s41598-017-16199-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,E) Effects of chalcones N9, N17 and N23 on the cell cycle. (A,D) histogram analysis of Red Fluor G1-positive cells; (E) Bar chart showing the percentage of positive HN30 cells stained with BrdU and treated with chalcones N9 (15.61 µM), N17 (14.27 µM) and N23 (17.22 µM), for 48 h; (F) Bar chart showing the percentage of HN30 on sub-G1 phase (red), G1 (white), S (light grey), G2/M (black) and >4 N (dark grey). Data represent the mean of 2 independent experiments performed in duplicate, and the lines indicate the standard error mean. Data were analyzed by one-way ANOVA, followed by Dunnet’s post-test; the group A represents the negative control. Significantly different from the control group (*P < 0.05); (**P < 0.01).