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. 2017 Nov 20;7:15850. doi: 10.1038/s41598-017-16199-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A,F) Analysis of gene expression profile of quinoxalinic chalcones (N9, N17 and N23) using PCR array. Scatter plots show the up-regulation (red dots) or the down-regulation (green dots) of genes related to cancer-related pathways (A – chalcone N9; B – chalcone N17; C – chalcone N23) and NFκB-mediated signal transduction (D chalcone N9; E – chalcone N17; F – chalcone N23) using real-time PCR via ‘Human Cancer PathwayFinder PCR Array’ and ‘Human NFκB Signaling Pathway plus PCR Array’. Changes in gene expression were calculated using the 2^-ΔCt method (RT²Profiler PCR array Data Analysis v3.5 - SABio/QIAGEN), and five stably expressed housekeeping genes (B2M, Hprt1, Rpl13α, Gapdh, and β-actin) were used for normalization of the results.