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. 2017 Nov 20;7:15856. doi: 10.1038/s41598-017-15977-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical Expression Pattern of Sox2 in aCP and Surrounding Brain Tissue. (a–c) Nuclear Sox2 staining (arrow) was found within aCP tumour bulk and palisading cell layer and in cells of the tumour surrounding brain tissue (asterisk; b,c,d). (e,f) Merged double-immunofluorescence staining of Sox2 (green) and the glial marker GFAP (red) demarcating adjacent brain tissue (asterisk) of the tumour revealed Sox2+ cells being enhanced nearby the CNS-tumour junction. (f) The GFAP negative tumour tissue showed also Sox2+ cells (arrow). (ada27 = a, ada56 = b, ada48 = c,d,e; ada50 = f).