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. 2017 Nov 20;7:15856. doi: 10.1038/s41598-017-15977-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Varying Amounts of Sox9 Expression in aCP and Surrounding Brain Tissue. (a,b) Some cases showed a higher level of nuclear Sox9 expression in both tumour and surrounding brain tissue indicated by an asterisk [a = ada56 (TIS 12, BT 3), b = ada49 (TIS 12, BT 3)], whereas others (c,d) were predominantly stained in just one of the two. (c) ada28: Low Sox9 expression within the tumour tissue (TIS 3), distinct expression in the surrounding brain (BT 3). (d) ada27: High Sox9 expression scores within the tumour (TIS 12) and only occasional nuclear staining within the surrounding brain tissue (BT 1). (e) Merged double immunofluorescence staining of ada48 underscored enhanced levels of Sox9+ cells (green) within the tumour adjoining GFAP+ (red) brain tissue (asterisk). TIS = total immunostaining score, BT = staining score within tumour surrounding brain tissue.