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. 2017 Nov 20;7:15856. doi: 10.1038/s41598-017-15977-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation of Sox9 and β-catenin Expression in Paraffin-embedded Serial Sections and Double-immunofluorescence Staining of aCP. Immunohistochemical staining of serial sections using antibodies against Sox9 (a) and β-catenin (b) showed the absence of Sox9 in cell clusters with nuclear β-catenin accumulations (*); Examples taken from ada47. Merging double-immunofluorescence staining with antibodies against Sox9 and β-catenin (c), we were able to confirm the previous observation that cell clusters with nuclear β-catenin accumulations (green, asterisk) lack Sox9 expression (red, asterisk); Example was taken from ada50.