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. 2017 Nov 20;7:15842. doi: 10.1038/s41598-017-15879-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the fabricated scaffolds in vitro. (A) The microstructures of PLGA-GO scaffolds (a1) and PLGA scaffolds (a2) under the scanning electron microscope. (B) Raman spectra of GO, pristine PLGA film and PLGA-GO film. (C) Under the 10 mJ pulsed laser stimulating, graph (c1) showed the PA signal producing by PLGA-GO scaffolds; graph (c2) showed the PA signal of PLGA-GO scaffolds with different intensities of Laser. (D) Scanning electron microscopy images showed that BMSCs attached and spread well on the surface of PLGA-GO scaffold after cultivating 72 h. White arrows indicated the BMSCs. (E) Proliferation tests of BMSCs growing on PLGA-GO scaffolds with or with PA stimulation at 24 h and 48 h. All quantitative data were presented as mean ± SD, n = 4. No statistical difference was found between group as indicated by unpaired two-tailed Student’s t test.