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. 2017 Nov 16;8:2260. doi: 10.3389/fmicb.2017.02260

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Copyright © 2017 Getino, Palencia-Gándara, Garcillán-Barcia and de la Cruz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of the PifC-mediated FI of R388 by overexpression of the coupling protein. Each point represents the conjugation frequency (T/D) of one independent experiment, in the presence or absence of pOX38 and R388 coupling protein TrwB. Horizontal and vertical bars represent the mean ± SD obtained for each group of data (^***p < 0.001). None/- (indicated by yellow circles), R388 alone. None/pBAD33::trwB (yellow circles), R388 in the presence of pBAD33::trwB. pOX38/- (blue squares), R388 in the presence of pOX38::KmR. pOX38/pBAD33 (blue squares), R388 in the presence of pOX38::KmR and pBAD33 empty vector. pOX38/pBAD33::trwB (orange squares), R388 in the presence of pOX38::KmR and pBAD33::trwB.